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Agarose and acrylamide powders, buffers, diluents, markers and stains for protein/nucleic acid electrophoresis; featuring concentration, grade, melting point, capacity to stabilize, control concentration, denature, reduce, and mark target molecules.
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Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
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HyperLadder 1kb is a popular ready-to-use molecular weight marker, especially designed for easy size determination. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder&trade 1kb.
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pI Markers, pI Value 8.79 *Markers for pI determination that will give you both consistent and accurate measurement of isoelectric point on your iCE system. *Can be used for whole-column and single-point capillary isoelectric focusing (cIEF) detection to measure your sample protein's isoelectric point, and for quality control of sample analysis. *You'll get 200 L of pI marker stock solution in every vial.
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The SuRE/Cut Buffer System consists of five optimized incubation buffers 10x concentrated for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.
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GelGreen is a sensitive stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (figure 1). Thus GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (figure 2). Unlike SYBR dyes which are known to be unstable GelGreen is very stable both hydrolytically and thermally.The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization under which GelGreen is not classified as hazardous waste.Spectral PropertiesAbsorbance Standard Transilluminator (254 nm) such as a Dark ReaderEmission Lon
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Designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.Vivaspin(R) sample concentrators are designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane leaving a more concentrated sample in the upper chamber.Vivaspin(R) sample concentrators cater for sample volumes from 100 mul to 20 ml with a range of molecular weight cutoff values from Mr 3 000 to 100 000. for maximum recovery select a MWCO at
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RNA MAX Buffer (20 ml). For use with the ZR small-RNA™ PAGE Recovery Kit. Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
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The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.
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